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Endoderm is the innermost of the three primary germ layers in the very early embryo. The other two layers are the ectoderm (outside layer) and mesoderm (middle layer). Cells migrating inward along the archenteron form the inner layer of the gastrula, which develops into the endoderm.
The endoderm consists at first of flattened cells, which subsequently become columnar. It forms the epithelial lining of multiple systems.
In plant biology, endoderm corresponds to the innermost part of the cortex (bark) in young shoots and young roots often consisting of a single cell layer. As the plant becomes older, more endoderm will lignify.